KMID : 1005220160310020123
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Journal of Embryo Transfer 2016 Volume.31 No. 2 p.123 ~ p.129
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Effect of Insulin Supplement on Development of Porcine Parthenogenetic Embryos
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Yu Young-Kwang
Roy Pantu Kumar Min Kyu-Hong Na Seung-Won Lee Eun-Cheol Kim Ghang-Yong Fang Xun Salih MB Cho Jong-Ki
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Abstract
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This study is performed to evaluate the effect of insulin in the porcine parthenogenetic embryo development. In porcine embryo culture, insulin is helpful factor in the process of embryo development. To identify this, insulin is used in pig embryos development. Therefore, this study was performed to investigate the effect of insulin on early embryonic development in pigs. For that, insulin positive or negative (0, 10 ug/mL) was supplemented in the porcine IVM media and then compared two groups divided by the cytoplasm of the black groups and white ring groups based on the distribution of lipid material of the cell cytoplasm in microscope. In maturation rates of porcine oocytes, significant higher black group rates were shown in the insulin positive groups compared with other groups (56.0¡¾2.1 vs 46.2¡¾0.3). In the embryo culture, black groups were showed the significant higher cleavage rates (82.1¡¾0.8, 78.3¡¾0.1 vs 63.2¡¾0.3, 63.4¡¾0.0), and blastocyst formation rates (15.5¡¾3.6, 16.6¡¾0.4 vs 11.7¡¾1.3, 7.4¡¾0.2) regardless of whether the addition of insulin. Also, black groups were showed higher cell number of blastocyst (33.2¡¾2.5, 35.5¡¾2.6 vs 31.2¡¾2.1, 31.3¡¾2.2). In conclusion, supplement of insulin producing black group in vitro maturation, it was effective in vitro maturation and embryonic development of pig embryos.
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KEYWORD
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Porcine , Embryo , in vitro production , Insulin
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